Metabolomic signatures for liver tissue and cecum contents in high-fat diet-induced obese mice based on UHPLC-Q-TOF/MS

Abstract Background The incidence of obesity is increasing worldwide, and it a risk factor for diabetes, dyslipidemia, nonalcoholic fatty liver disease. Our previous study had demonstrated that high-fat diet induced increased weight gain, fat weight, serum cholesterol, triglyceride, ATL levels in liver, influenced the diversity composition cecal microbiota mice. Hence, this aimed to investigate roles gut microbially derived metabolites between obese lean mice, focusing on their association with progression by (HFD). Methods An model mice was established HFD 16 weeks. Cecal contents tissues metabolomics based ultraperformance liquid chromatography-quadrupole-time-of-flight mass spectrometry orthogonal partial least squares discriminant analyses (OPLS-DA) performed identify alterations associated Results Obese groups were clearly discriminated from each other OPLS-DA score plot major contributing discrimination mainly involved glycerophospholipid metabolism, primary bile acid biosynthesis, biosynthesis unsaturated acids pathways. HFD-induced 19 43 cecum identified as potential biomarkers related obesity. Specifically, chenodeoxycholic acid, taurochenodeoxycholate, tauroursodeoxycholic elevated 35.94, 24.36, 18.71-fold, respectively. PI(P-16:0/18:1(9Z)), PG(19:0/16:0), PS(P-16:0/20:2(11Z,14Z)), PI(22:1(11Z)/12:0), PE(21:0/0:0) enhanced 884, 640.96, 226.63, 210.10, 45.13-fold comparison These most important discriminating In addition, strongly correlated hepatic through gut-liver axis analysis. Conclusions lipid profiles (i.e. glycerophospholipids, PC, PE, PI, PG, PS) total (primary secondary acid) cecum, suggesting they may play an role can be used better understand disease HFD. Furthermore, level these assess therapeutic effect management.